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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is using CRISPR-based technology for rapid and sensitive detection of specific bacterial DNA sequences directly from patient samples. This method relies on the ability of the Cas enzyme to:

A) Detect the presence of specific antibodies produced in response to bacterial infection.
B) Specifically bind to and cleave target DNA sequences guided by a short RNA molecule.
C) Reverse transcribe RNA into complementary DNA.
D) Amplify target DNA sequences using specific primers and thermal cycling.

2. Lyme disease, characterized initially by erythema migrans, is caused by Borrelia burgdorferi. What is the primary arthropod vector responsible for transmitting this spirochete to humans in the Northeastern United States?

A) Amblyomma americanum (Lone Star tick)
B) Pulex irritans (Human flea)
C) Ixodes scapularis (Blacklegged tick)
D) Dermacentor variabilis (American dog tick)

3. A research team is investigating the mechanism of bacterial resistance to a novel fluoroquinolone antibiotic.
They isolate a resistant strain of Escherichia coli from a patient with a urinary tract infection. Genetic analysis of this strain reveals a point mutation in the gyrA gene. This mutation MOST likely results in:

A) Increased expression of efflux pumps that actively transport the antibiotic out of the cell.
B) Modification of the ribosomal target site, preventing antibiotic binding.
C) Alteration of the DNA gyrase enzyme, reducing its affinity for the fluoroquinolone.
D) Production of an enzyme that inactivates the fluoroquinolone molecule.

4. A traveler returning from a tropical region presents with fever, chills, and jaundice. Peripheral blood smear reveals the presence of ring-shaped parasites within erythrocytes. Further laboratory testing shows a positive result for Plasmodium falciparum histidine-rich protein 2 (HRP2). The MOST appropriate treatment for this patient is:

A) Amphotericin B
B) Mebendazole
C) Primaquine and a blood schizonticide (e.g., artemisinin-based combination therapy)
D) Metronidazole

5. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Increased expression of efflux pumps.
B) Production of beta-lactamase enzymes.
C) Acquisition of genes encoding aminoglycoside-modifying enzymes.
D) Alteration of penicillin-binding proteins (PBPs).

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